Bismark report for: ../SRR1258894_2M_1.fastq and ../SRR1258894_2M_2.fastq (version: v0.19.0) Bismark was run with Bowtie against the bisulfite genome of /pbld/netapp/home/dslee/Refs/lamda/ with the specified options: -q -n 1 -k 2 --best --maxins 500 --chunkmbs 512 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 2000000 Number of paired-end alignments with a unique best hit: 4020 Mapping efficiency: 0.2% Sequence pairs with no alignments under any condition: 1995980 Sequence pairs did not map uniquely: 0 Sequence pairs which were discarded because genomic sequence could not be extracted: 14 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2202 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 1818 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 211690 Total methylated C's in CpG context: 150 Total methylated C's in CHG context: 176 Total methylated C's in CHH context: 337 Total unmethylated C's in CpG context: 54691 Total unmethylated C's in CHG context: 56870 Total unmethylated C's in CHH context: 99466 C methylated in CpG context: 0.3% C methylated in CHG context: 0.3% C methylated in CHH context: 0.3% Bismark completed in 0d 0h 0m 48s